Momwe mungasankhire mbale yoyenera ya ELISA?

Momwe mungasankhire mbale yoyenera ya ELISA?

Maonekedwe a pansi
Pansi Pansi: Pansi ndi yopingasa, yomwe imatchedwanso F pansi.Kuwala kodutsa pansi sikudzasokonekera, ndipo kufalikira kwa kuwala kumatha kukulitsidwa.Amagwiritsidwa ntchito pazoyesera zomwe zimafunikira pansi mozungulira kuti ziwonekere kapena zifukwa zina.
Pansi Pansi: Amatchedwanso U-bottom, amapereka kuyeretsa koyenera komanso kusakaniza kwa ntchito zomwe zimafuna kuyesa matope.
C-Pansi: Pakati pa pansi lathyathyathya ndi pansi mozungulira kupereka zotsatira zabwino zoyeretsera ndi kuphatikiza ubwino wa pansi lathyathyathya.
Pansi pa Cone: Imadziwikanso kuti V pansi, ndiyoyenera kusanja bwino ndikusunga zitsanzo zazing'ono kuti mubwezeretsenso ma volume ang'onoang'ono.
Mtundu
Ambiri a ELISA amasankha mbale zowonekera ngati zinthu zoyesera.Mambale oyera ndi akuda nthawi zambiri amagwiritsidwa ntchito pozindikira luminescence.Ma mbale akuda a ELISA ali ndi kuyamwa kwawo kowala, kotero chizindikiro chawo ndi chofooka kuposa mbale zoyera za ELISA.Nthawi zambiri mbale zakuda zimagwiritsidwa ntchito pozindikira kuwala kolimba, monga kuzindikira kwa fluorescence;M'malo mwake, mbale zoyera zitha kugwiritsidwa ntchito pozindikira kuwala kocheperako, ndipo zimagwiritsidwa ntchito ngati chemiluminescence komanso kakulidwe kamtundu wa gawo lapansi (mwachitsanzo, kusanthula kwa majini a mtolankhani wapawiri-luciferase).
Zakuthupi
Zida wamba ndi polyethylene, Pe, polypropylene, PP, Polystyrene, PS, Polyvinylchloride, PVC, Polycarbonate, PC.
Zida zomwe zimagwiritsidwa ntchito kwambiri mu ELSIA ndi polystyrene ndi polyvinyl chloride.Polyvinyl chloride ndi yofewa, yopyapyala, yodulidwa komanso yotsika mtengo.Choyipa chake ndikuti kumaliza kwake sikuli bwino ngati mapepala a polystyrene ndipo pansi pa dzenje silathyathyathya ngati polystyrene.Komabe, pali kuwonjezereka kofananirako kwamakhalidwe akumbuyo.Kawirikawiri, pamwamba pa enzyme yolembera mbale iyenera kuthandizidwa ndi ionic grafting, yomwe imayambitsa magulu ogwira ntchito monga aldehyde, gulu la amino ndi gulu la epoxy pamwamba pa polima kuti apititse patsogolo ntchito ya gawo lapansi.
Njira zomangira zosiyanasiyana
Kumanga mogwira mtima kwa chinthu chotsekedwa pansi