Kodi machubu a ultrafiltration centrifuge angagwiritsidwenso ntchito?Nali yankho

Chubu cha centrifuge ndi chubu chosavuta chomwe chimatha kupirira kuthamanga kwambiri komanso kuthamanga, monga kulekanitsa zitsanzo ndi kulekanitsa matope apamwamba kwambiri.The ultrafiltration centrifuge chubu ili ndi magawo awiri ofanana ndi chubu lamkati ndi chubu lakunja.Chubu chamkati ndi nembanemba yokhala ndi kulemera kwina kwa molekyulu.Panthawi ya centrifugation yothamanga kwambiri, omwe ali ndi kachuluke kakang'ono ka molekyulu amathamangira mu chubu chapansi (ie chubu lakunja), ndipo omwe ali ndi kulemera kwakukulu kwa maselo amatsekeredwa mu chubu chapamwamba (ie chubu lamkati).Iyi ndiye mfundo ya ultrafiltration ndipo nthawi zambiri imagwiritsidwa ntchito kuyika zitsanzo.

Ultrafiltration centrifuge machubu nthawi zambiri angagwiritsidwe ntchito popanda pretreatment, koma mapuloteni chitsanzo processing, makamaka kuchepetsa mapuloteni (<10ug / ml), kuchira mlingo wa ndende ndi ultrafiltration nembanemba nthawi zambiri si kuchuluka.Ngakhale zida za PES zimachepetsa kudsorption mosadziwika bwino, mapuloteni ena, makamaka akakhala ocheperako, amatha kukhala ndi zovuta.Mlingo wa kumangiriza kosafunikira kumasiyanasiyana malinga ndi momwe mapuloteni amapangidwira.Mapuloteni okhala ndi madomeni ochajitsidwa kapena opangidwa ndi hydrophobic amatha kumangika mosasinthika kumalo osiyanasiyana.Passivation pretreatment pamwamba pa chubu cha ultrafiltration centrifuge chingachepetse kutayika kwa mapuloteni adsorption pamtunda.Nthawi zambiri, pretreatment of the column isanakhazikitse njira yochepetsera mapuloteni imatha kuwongolera kuchuluka kwa kuchira, chifukwa yankho limatha kudzaza malo opanda kanthu a protein adsorption powonekera pa nembanemba ndi pamwamba.Njira yopititsira patsogolo ndikuyika gawolo ndi njira yopitilirapo yopitilira ola la 1, kutsuka ndimeyo bwino ndi madzi osungunuka, kenako ndikuyiyika kamodzi ndi madzi osungunuka kuti ichotseretu yankho lomwe lingakhale pafilimuyo. .Samalani kuti musalole kuti filimuyo iume pambuyo pa passivation.Ngati mukufuna kugwiritsa ntchito pambuyo pake, muyenera kuwonjezera madzi osabala osungunuka kuti filimuyo ikhale yonyowa.

Machubu a Ultrafiltration centrifuge nthawi zambiri sangatsekedwe ndikugwiritsidwanso ntchito.Popeza mtengo wa chubu limodzi ndi wotsika mtengo, anthu ambiri amayesa kugwiritsiranso ntchito - zomwe zimachitikira ndikuyeretsa pamwamba pa membrane ndi madzi osungunuka nthawi zambiri ndi centrifuge kamodzi kapena kawiri.Kachubu kakang'ono kamene kamatha kukhala centrifuged m'mbuyo akhoza kumizidwa m'madzi osungunuka ndiyeno centrifuged mosinthana nthawi zambiri, zomwe zingakhale bwino.Ikhoza kugwiritsidwa ntchito mofananamo mobwerezabwereza, ndipo ikhoza kuviikidwa m'madzi osungunuka pamene sichikugwiritsidwa ntchito, koma kuipitsidwa ndi bakiteriya kudzapewedwa.Osasakaniza zitsanzo zosiyanasiyana.Anthu ena amati kuthira mowa wa 20% ndi 1n NaOH (sodium hydroxide) kungalepheretse kukula kwa mabakiteriya ndikuletsa kuyanika.Malingana ngati nembanemba ya ultrafiltration ilowa m'madzi, singaloledwe kuti iume.Komabe, ena amanena kuti idzawononga dongosolo la nembanemba.Mulimonsemo, opanga nthawi zambiri samathandizira kugwiritsa ntchitonso.Kugwiritsiridwa ntchito mobwerezabwereza kudzalepheretsa kukula kwa pore kwa nembanemba ya fyuluta, komanso kuchititsa kuti madzi azituluka, zomwe zingakhudze zotsatira zoyesera.