Njira yolondola yogwiritsira ntchito ndi masitepe a serological pipette

Pipette ya serological, yomwe imadziwikanso kuti pipette yotayika, imagwiritsidwa ntchito makamaka poyesa molondola kuchuluka kwa madzi, omwe ayenera kugwiritsidwa ntchito pamodzi ndi pipette yoyenera.Pipette ndi chipangizo choyezera chomwe chimagwiritsidwa ntchito kusamutsa molondola kuchuluka kwa yankho.Pipette ndi chida choyezera, chomwe chimangogwiritsidwa ntchito poyesa kuchuluka kwa yankho lomwe limatulutsa.Ndi chubu chagalasi chachitali komanso chopyapyala chokhala ndi kukulitsa kwakukulu pakati.Mapeto ake apansi ndi mawonekedwe a pakamwa lakuthwa, ndipo khosi lapamwamba la chitoliro limalembedwa ndi mzere wolembera, womwe ndi chizindikiro cha voliyumu yeniyeni yomwe iyenera kusuntha.

Njira yoyenera yogwiritsira ntchito ndi masitepe a serum pipette:

1. Musanagwiritse ntchito: Mukamagwiritsa ntchito pipette, choyamba yang'anani chizindikiro cha pipette, mlingo wolondola, malo a chizindikiro, ndi zina zotero.

2. Aspiration: Gwirani kumtunda kwa pipette ndi chala chachikulu ndi chapakati cha dzanja lanu lamanja, ndikuyika pakamwa pamunsi pa pipette mu njira yothetsera kuyamwa.Kuyikapo sikuyenera kukhala kozama kwambiri kapena kuzama, nthawi zambiri 10 ~ 20mm.Ngati ili yozama kwambiri, imatha kuyamwa.Kulakalaka yankho mu mpira wotsuka makutu kumayipitsa yankho.Ngati yazama kwambiri, imamatira njira yochulukirapo kunja kwa chubu.Tengani mpira wosambitsa khutu ndi dzanja lamanzere, gwirizanitsani kukamwa kumtunda kwa chubu ndikupuma pang'onopang'ono yankho.Choyamba, lowetsani pafupifupi 1/3 ya voliyumu ya chubu.Dinani pakamwa pa chubu ndi chala chakumanja chakumanja, chitulutseni, chigwireni mopingasa, ndikuzungulira chubu kuti yankho ligwirizane ndi gawo lomwe lili pamwamba pa sikelo kuti lilowe m'malo mwa madzi pakhoma lamkati.Kenako tulutsani yankho kuchokera m'munsi pakamwa pa chubu ndikutaya.Mukatsuka mobwerezabwereza katatu, mutha kuyamwa njira yofikira pafupifupi 5mm pamwamba pa sikelo.Nthawi yomweyo akanikizire chubu pakamwa ndi cholozera chala cha dzanja lamanja.

3. Sinthani mlingo wamadzimadzi: kwezani pipette mmwamba ndi kutali ndi mlingo wamadzimadzi, pukutani madziwo pakhoma lakunja la pipette ndi pepala la fyuluta, mapeto a chubu amatsamira pa khoma lamkati la chidebe cha yankho, chubu. thupi amakhala ofukula, kumasuka pang'ono cholozera chala kuti yankho mu chubu pang'onopang'ono kutuluka m'kamwa m'munsi, mpaka pansi pa meniscus ya yankho ndi tangent kwa cholemba, ndipo nthawi yomweyo akanikizire chubu pakamwa ndi cholozera chala.Chotsani dontho lamadzi pakhoma, lichotseni ku pipette, ndikuyiyika muchombo cholandira yankho.

4. Kutulutsa yankho: Ngati chotengera cholandirira yankho ndi botolo la conical, botolo la conical liyenera kupendekeka 30 °.Pipette yotayika iyenera kukhala yoyima.Mapeto apansi a chubu ayenera kukhala pafupi ndi khoma lamkati la botolo la conical.Masulani chala cholozera ndikulola yankho liyende pang'onopang'ono pansi pa khoma la botolo.Pamene mlingo wamadzimadzi umatsikira kumutu wotuluka, chubucho chimakhudza khoma lamkati la botolo kwa masekondi pafupifupi 15, ndikuchotsa pipette.Njira yaying'ono yomwe yatsala kumapeto kwa chubu sayenera kukakamizidwa kutuluka, Chifukwa kuchuluka kwa yankho losungidwa kumapeto kwawerengedwa.