Kodi mwaphunzira mulingo wosindikiza filimu?
Chani?Ndani winanso sangathe "kusindikiza filimu"?Yankhani mwachangu nkhaniyi kuti ikuphunzitseni "filimu yosindikiza" yolondola!
Zoonadi, "filimu yosindikizira" apa ndikusindikiza mbale ya 96 bwino ya PCR kuti muwonetsetse kuti filimu yosindikizira ikugwirizana kwambiri ndi mbale ya 96 ndikuletsa kutuluka kwamadzimadzi, kuti muwonetsetse kuyesa kosalala.
1. Mamata filimu yosindikiza pa bolodi
Tulutsani chikwama chimodzi chosindikizira kuchokera muthumba lodzisindikizira, ndikusindikizanso thumba lodzisindikiziralo kuti ma enzyme asakhale malo.Pitirizani kuyang'ana pansi, gwirani filimu yosindikizira, ndipo pang'onopang'ono mugwetse chinsalu chapansi motsatira mzere wozungulira.
Kenako, gwirani mbali imodzi ya zomatira pamwamba pa filimu yosindikiza pa bolodi, ndipo gwirani mtunda ndi ngodya kuti mupewe skew yotsatira.M’kati mwa phala, mbali imodzi imaikidwa ndipo ina imakoka.
Langizo: Valani magolovesi nthawi zonse
● Ngati filimu yosindikizira ya chizindikiro chimodzi chokha chikugwiritsidwa ntchito, chotsani chingwecho pang'onopang'ono, nangula filimu yosindikizira pa bolodi kuti musindikize pa bolodi lonse, ndiyeno pitirizani kuchotsa chingwecho.Njirayi imatha kuthetsa kupindika ndi kubwezeretsanso chifukwa cha filimu yosindikiza.
● Ngati mankhwala okhala ndi zilembo ziwiri agwiritsidwa ntchito, chotsani chingwe chapakati mosalekeza komanso mosalala.Kudula pang'onopang'ono kumachepetsa kufota.Samalani kuti musakhudze kugwirizana pamwamba pa filimuyo.
2. Kukanikiza filimu
Gwiritsani ntchito mbale yopondereza kuti mufufuze pang'onopang'ono ndikusindikiza filimu yosindikizira kuti ikhale yosindikizidwa pa mbale.Ngati palibe laminates apadera, mungapeze khadi yokhala ndi m'mphepete mwake, monga khadi la banki kapena khadi la basi.
filimu kukanikiza sitepe ayenera kuchitidwa osachepera kawiri horizontally ndi vertically.Ndikofunikira kugwiritsa ntchito mphamvu zokwanira kuti mupeze chisindikizo chabwino.
Pala ndi kusindikiza mbale yosindikizira ya nembanemba m'mbali zonse zakunja kwa mbale ya orifice osachepera kawiri kuti muwonetsetse kuti kukakamiza kolimba ndi kosalekeza kukugwiritsidwa ntchito.Mabowo ndi m'mphepete adzapanikizidwa kamodzi.Mukasindikiza filimu yosindikizira bwino pa mbale, chotsani gawo lolumikizana ndi mzere wa tangent.
Langizo: ● Mukakanikiza filimuyo, gwirani bolodi ndi dzanja lina kuti musagwedezeke mwamphamvu bolodi.
3. Kuyendera
Mukasindikiza, yang'anani mosamala mbale yathyathyathya kuti mutsimikizire ngati filimuyo ili pafupi kwambiri ndi mbaleyo.Tsimikizirani kuti zomatira kuzungulira dzenje lililonse, mbali yonse ya mbale (kuphatikiza periphery) imasindikizidwa, komanso ngati pali madzi pa nembanemba.Filimu yosindikiza sayenera kukhala ndi makwinya.Ngati makwinya awonedwa, mbaleyo siinasindikizidwa bwino.
● Kwa mbale zathyathyathya zokhala ndi m'mphepete mwake, kuyimitsidwa kwa filimu yosindikizira pa mbale sikungakhale kolondola, ndipo filimuyo isapitirire mmwamba ku khoma lakumbali la mbale.
Ikani mbale yosindikizidwa kwa mphindi zosachepera 10 musanayambe kuyesa kwa PCR, ndipo mphamvu yomatira ya filimu yosindikiza idzawonjezeka ndi nthawi.Ngati n'kotheka, gwiritsani ntchito centrifuge yapadera ya orifice plate pothandizira centrifugation.Pomaliza, tumizani mbale yosindikizidwa ku makina a PCR kuti muyambe kuyesa ~
Langizo:
● Kwa mbale zathyathyathya zokhala ndi m'mphepete mwake, kuyimitsidwa kwa filimu yosindikizira pa mbale sikungakhale kolondola, ndipo filimuyo isapitirire mmwamba ku khoma lakumbali la mbale.
Ikani mbale yosindikizidwa kwa mphindi zosachepera 10 musanayambe kuyesa kwa PCR, ndipo mphamvu yomatira ya filimu yosindikiza idzawonjezeka ndi nthawi.Ngati n'kotheka, gwiritsani ntchito centrifuge yapadera ya orifice plate pothandizira centrifugation.Pomaliza, tumizani mbale yosindikizidwa ku makina a PCR kuti muyambe kuyesa ~
Nthawi yotumiza: Dec-16-2022