Momwe mungasankhire moyenera kugwiritsa ntchito chikhalidwe cha cell "mabotolo, mbale ndi mbale"?

Momwe mungasankhire moyenera kugwiritsa ntchito chikhalidwe cha cell "mabotolo, mbale ndi mbale"?

Polima ma cell, nthawi yoti mugwiritse ntchito ziboliboli zachikhalidwe komanso nthawi yogwiritsira ntchito mbale zachitsime zimatengera cholinga ndi zosowa za kuyesako.Kawirikawiri, ma flasks a chikhalidwe cha ma cell amagwiritsidwa ntchito pa chikhalidwe choyambirira cha maselo ndi chikhalidwe chodziwika bwino, ndipo maselo ambiri oyesera amatha kupezeka.

Mabotolo a chikhalidwe cha ma cell amapangidwa ndi zinthu zapamwamba kwambiri za polystyrene (PS), zopangidwa ndi nkhungu zolondola kwambiri komanso njira zopangira zokha.Zogulitsazo zimagwiritsidwa ntchito mu chikhalidwe cha ma laboratory cell.Mawonekedwe awo abwino kwambiri amathandizira kuwunika kwapang'onopang'ono.Pamwambapo adathandizidwa ndi TC kuti awonetsetse kuti ma cell amamatira.Zotsatira zabwino.

1) Momwe mungasankhire ma flasks a chikhalidwe ndi mbale za chikhalidwe ku maselo a chikhalidwe

Choyamba, sankhani potengera kuchuluka kwa maselo omwe akuyembekezeka.

Kachiwiri, sankhani potengera luso la ntchito zoyeserera.Kaya ndikusintha ma media, njira, kapena kukolola ma cell, kugwiritsa ntchito mbale zachikhalidwe ndikosavuta, koma chifukwa chakutseguka kwake, ndikosavuta kuyipitsa.

2) Ndibwino kugwiritsa ntchito mbale zamtundu wa selo zoyesera zomwe zimagwiritsa ntchito maselo monga zonyamulira kapena zinthu, monga kuyesa kutengeka kwa mankhwala, MTT (96-well culture plate), immunohistochemistry (6-well culture plate), etc.