Momwe mungasankhire "chubu chozizira" chabwino kwambiri?

Momwe mungasankhire "chubu chozizira" chabwino kwambiri?

Chosavuta kugwiritsa ntchito chubu cha cryo sichingangokwaniritsa zofunikira zoyeserera, komanso kuchepetsa mwayi wangozi zoyeserera pamlingo wina.

Lero tigwiritsa ntchito njira zitatu posankha chubu cha cryo.

Gawo loyamba: zakuthupi

Monga tonse tikudziwira, machubu oziziritsa amagwiritsidwa ntchito makamaka pamayendedwe otsika kutentha ndi kusungirako minyewa kapena zitsanzo zama cell, nthawi zambiri muzofufuza zamankhwala ndi zamankhwala.

Chifukwa chubu chozizira chimalumikizana mwachindunji ndi chitsanzo, choyamba ndikusankha zinthu zoyenera kuti musaipitsidwe ndi chitsanzocho.

Nthawi zambiri, machubu oziziritsa amapangidwa ndi zinthu zopanda cytotoxicity.Zida zomwe zimagwiritsidwa ntchito kwambiri m'ma laboratories ndi pulasitiki ndi magalasi.Komabe, chifukwa ma cryotubes agalasi sangathe kugwiritsidwa ntchito pama centrifuges othamanga kwambiri kapena othamanga kwambiri, ma cryotube apulasitiki amagwiritsidwa ntchito.

Pali zinthu zambiri zamapulasitiki, mungasankhe bwanji?

Mawu asanu, "polypropylene chuma" Sankhani ndi chidaliro!

Polypropylene imakhala ndi kukhazikika kwamankhwala komanso kutentha.Pansi pa mpweya wa nayitrogeni wamadzimadzi, imatha kupirira kutentha kochepa mpaka 187 ℃.

Kuonjezera apo, ngati zofunikira pachitetezo chachitsanzo ndizokwera kwambiri, zinthu zopanda mutagenic ndi machubu ogwirizana a VID a pyrogen akhoza kusankhidwa.Ndipo chonde musatsegule musanagwiritse ntchito.Ngati yatsegulidwa kale, iyenera kutsekedwa musanagwiritse ntchito!

Gawo lachiwiri: Kupanga

Chubu chozizira nthawi zambiri chimapangidwa ndi chipewa cha chubu ndi thupi la chubu, chomwe chimagawidwa kukhala chubu chozizira chamkati ndi kapu yakunja yoziziritsa.Ngati chitsanzocho chiyenera kusungidwa mu gawo la nayitrogeni yamadzimadzi, gwiritsani ntchito chubu chozizira chamkati chokhala ndi silika gel osakaniza;Ngati chitsanzocho chiyenera kusungidwa mu zipangizo zamakina, monga firiji, chubu chozizira chozungulira chakunja chimagwiritsidwa ntchito, nthawi zambiri popanda silika gel pad.

M'mawu amodzi:

Pazonse, kutsika kwa kutentha kwa chubu chamkati chozungulira cryopreservation ndikwabwino kuposa chubu chozizira chakunja, chomwe chiyenera kusankhidwa molingana ndi zosowa zenizeni.

Gawo lachitatu: zofotokozera

Malinga ndi zoyeserera, machubu osungira nthawi zambiri amakhala ndi 0.5ml, 1.0ml, 2.0ml, 5ml, ndi zina zambiri.

Machubu oziziritsa achilengedwe omwe amagwiritsidwa ntchito kwambiri nthawi zambiri amakhala 2ml kukula kwake.Kuyenera kudziwidwa kuti voliyumu ya chitsanzo nthawi zambiri sangapitirire magawo awiri pa atatu a voliyumu ya kuzizira kwa chubu.Choncho, chubu choyenera chozizira chiyenera kusankhidwa molingana ndi kukula kwa chitsanzo chozizira

Komanso, pali kusiyana pawiri wosanjikiza ndi sanali awiri wosanjikiza, akhoza kukhazikitsidwa ndipo sangathe kukhazikitsidwa, zoweta ndi kunja, ndi mtengo.Izi ndi zinthu zomwe ziyenera kuganiziridwa posankha chubu chozizira.