Momwe mungasankhire mbale ya PCR kuti mugwiritse ntchito mu labotale?
Ma mbale a PCR nthawi zambiri amakhala 96-bowo ndi 384-bowo, kutsatiridwa ndi 24-bowo ndi 48-bowo.Chida cha PCR chomwe chikugwiritsidwa ntchito komanso momwe pulogalamuyo ikugwiritsidwira ntchito zidzatsimikizira ngati PCR board ndiyoyenera kuyesa kwanu.Kotero, momwe mungasankhire bolodi la PCR la zinthu za labotale molondola?
1, Mitundu yosiyanasiyana ya siketi ilibe matabwa a siketi ndipo ilibe mapanelo ozungulira.
Mtundu uwu wa mbale zomwe zingasinthidwe ndi zigawo zambiri za zida za PCR ndi zida zenizeni za PCR, koma sizoyenera kugwiritsa ntchito zokha.
Siketi ya siketi ya theka imakhala ndi m'mphepete mwaifupi m'mphepete mwa mbale ndipo imapereka chithandizo chokwanira panthawi yotumiza madzi.Zida zambiri za Applied Biosystems PCR zimagwiritsa ntchito mbale za siketi ya theka.
Bolodi ya PCR yokhala ndi siketi yonse ili ndi gulu la m'mphepete lomwe limaphimba kutalika kwa bolodi.Gulu lamtunduwu ndiloyenera chida cha PCR chokhala ndi gawo lotuluka (lomwe limathandizira kuti lizigwira ntchito zokha), ndipo limatha kusinthidwa mosamala komanso mokhazikika.Siketi yathunthu imapangitsanso mphamvu zamakina, ndikupangitsa kuti ikhale yoyenera kwambiri kugwiritsa ntchito nsanja ya robot mumayendedwe oyenda okha.
2, Mitundu yosiyanasiyana yamagulu
Mapangidwe apansi athunthu amagwira ntchito pazida zambiri za PCR ndipo ndiosavuta kusindikiza ndi kukonza.
Kapangidwe ka mbale ka convex kamakhala ndi kusinthika kwabwino kwa zida zina za PCR (monga chida cha Applied Biosystems PCR), chomwe chimathandizira kukhazikika kwa chipewa cha kutentha popanda kufunikira kwa adaputala, kuwonetsetsa kutengera kutentha komanso zotsatira zodalirika zoyesera.
Ma mbale a PCR nthawi zambiri amatha kupereka mitundu yosiyanasiyana yamitundu kuti athandizire kusiyanitsa ndi kuzindikira zitsanzo, makamaka pakuyesa kwapamwamba.Ngakhale mtundu wa pulasitiki ulibe mphamvu pakukulitsa kwa DNA, tikulimbikitsidwa kugwiritsa ntchito zinthu za pulasitiki zoyera kapena zonyezimira za pulasitiki kuposa zowonekera poyika momwe ma PCR amtundu wa fluorescence amathandizira kuti akwaniritse kuzindikira komanso kulondola kwa fulorosenti.
4. Malo osiyana chamfer
Kudula pamakona ndi ngodya yosowa ya mbale ya PCR, zomwe zimadalira chida chomwe chiyenera kusinthidwa.Chamfer ikhoza kukhala pa H1, H12 kapena A12 ya 96-hole plate, kapena A24 ya 384-hole plate.
5, mtundu wa ANSI/SBS
Kuti ikhale yogwirizana ndi makina osiyanasiyana amadzimadzi ogwiritsira ntchito makina ochulukirachulukira, bungwe la PCR liyenera kutsatira zomwe bungwe la American National Standards Association (ANSI) ndi Society for Biological and Molecular Sciences (SBS), lomwe tsopano ndi logwirizana ndi Laboratory Automation ndi Screening Association (SLAS).Bolodi yogwirizana ndi ANSI/SBS ili ndi kukula kwake, kutalika, malo a dzenje, ndi zina zotero, zomwe zimathandiza pokonza zokha.
6. Mphepete mwa dzenje
Pali m'mphepete mwake mozungulira dzenjelo.Kapangidwe kameneka kangathandize kusindikiza ndi filimu yosindikizira kuti zisafufutike.
7, Marko
Nthawi zambiri imakhala chizindikiro chokwezeka cha alphanumeric chokhala ndi zolembera zoyera kapena zakuda mumtundu woyamba kuti muwone mosavuta.
Nthawi yotumiza: Feb-10-2023