Momwe mungagwiritsire ntchito chubu cha cryopreservation mwasayansi komanso molondola
Kugwiritsa ntchito chubu cha cryopreservation ndi sayansi, osati trilogy wamba monga kutsegula thanki ya nayitrogeni yamadzimadzi, kuyiyika mu chubu cha cryopreservation ndikutseka thanki yamadzi ya nayitrogeni.Kugwiritsa ntchito mwasayansi komanso kolondola kwa machubu oteteza ku cryopreservation kumatha kupewa kutayika kwa zitsanzo ndikuteteza chitetezo cha oyesa.
Kugwiritsa ntchito chubu cha cryopreservation ndi sayansi, osati trilogy wamba monga kutsegula thanki ya nayitrogeni yamadzimadzi, kuyiyika mu chubu cha cryopreservation ndikutseka thanki yamadzi ya nayitrogeni.Kugwiritsa ntchito mwasayansi komanso kolondola kwa machubu oteteza ku cryopreservation kumatha kupewa kutayika kwa zitsanzo ndikuteteza chitetezo cha oyesa.
Kuzizira chubu: masitepe oziziritsa
Sambani ma cell ndi preheated PBS solution, yamwani yankho, ndikuphimba ma cell ndi yankho lomwe lili ndi trypsin ndi EDTA (wosanjikiza wamadzi wochepa kwambiri ndi wokwanira, komanso kuchuluka kwa trypsin ndi EDTA kumafunika kutsimikiziridwa molingana ndi mzere wa cell).
Ikani ma cell pa 37 ℃ kwa mphindi 3-5.
Maselo akachoka pansi, makulitsidwe amatha, sing'anga yomwe ili ndi seramu imawonjezeredwa, ndipo maselo amayimitsidwa pang'onopang'ono ndi pipette.
Centrifuge kuyimitsidwa kwa cell (500 xg, 5 min) ndikuyimitsanso ndi sing'anga yomwe ili ndi seramu.
Chiwerengero cha ma cell.
Centrifuge kuyimitsidwa kwa cell (500 xg, mphindi 5), chotsani chapamwamba, ndikuyimitsanso ma cell ndi sing'anga yomwe ili ndi seramu ya voliyumu yoyenera.
Sakanizani ma cell ndi cryopreservation solution (60% medium, 20% fetal bovine serum, 20% DMSO) mu chiŵerengero cha 1:1 voliyumu, ndiyeno kuwasamutsira ku Cryo STM cryopreservation chubu.Kuchulukana kwa maselo oundana ndi 1-5 × 106 zidutswa / ml.
Cryo yomwe ili ndi ma cell STM cryopreservation chubu ikulimbikitsidwa kuti izizirike pamlingo wa - 1 K / min, ndi chubu cha cryopreservation chikhoza kuikidwa mu chidebe chokhala ndi isopropanol pa -70 ℃.Ngati Cryo STM cryopreservation chubu imasunga zitsanzo zina, zomwe zitha kuyikidwa mwachindunji pa − 20 ℃, − 70 ℃ kapena gawo la gasi la nayitrogeni wamadzimadzi.Kuonetsetsa kuti chitsanzocho chazizira mofanana, 4 mL ndi 5 mL Cryo The sTM cryopreservation chubu iyenera kuikidwa mufiriji pa − 20 ℃ kwa usiku wonse, ndiyeno imasamutsidwa ku gawo la mpweya - 70 ℃ kapena madzi asatrojeni.
Kenako sinthani Cryo.sTM cryopreservation chubu kupita ku thanki ya nayitrogeni yamadzimadzi.Kuti mupewe kuipitsa (monga mycoplasma) ndi malingaliro achitetezo, chonde ikani Cryo.sTM cryopreservation chubu mu gawo la mpweya wa nayitrogeni wamadzimadzi, osati mu gawo lamadzimadzi.
Momwe mungagwiritsire ntchito chubu cha cryopreservation mwasayansi komanso molondola?Kampani yathu imapangidwa ndi akatswiri omwe ali ndi mbiri yamakampani komanso odziwa zambiri zamsika kuti apereke zinthu zopangira kafukufuku wa sayansi ya moyo ndi ntchito kwa ofufuza asayansi.Sizingakwaniritse zofunikira zapadera za makasitomala a R&D pamitundu yazogulitsa ndi kuyika, komanso kukwaniritsa zofunikira zamabizinesi opanga pamagawo onse kuyambira ang'onoang'ono, apakati mpaka kupanga zazikulu.
Nthawi yotumiza: Nov-25-2022