Gulu la Petri mbale——
1. Malinga ndi ntchito zosiyanasiyana za chikhalidwe mbale, iwo akhoza kugawidwa mu mbale chikhalidwe cell ndi mabakiteriya chikhalidwe mbale.
2. Ikhoza kugawidwa mu mbale za pulasitiki za petri ndi mbale zagalasi za petri malinga ndi zipangizo zosiyanasiyana zopangira, koma mbale zambiri za petri zomwe zimatumizidwa kunja ndi zotayidwa za petri ndi zipangizo zapulasitiki.
3. Malingana ndi kukula kwake, amatha kugawidwa kukhala 35mm, 60mm ndi 90mm m'mimba mwake.150mm Petri mbale.
4. Malinga ndi kusiyana kwa kulekana, zikhoza kugawidwa mu 2 osiyana Petri mbale, 3 osiyana Petri mbale, etc.
5. Zida za mbale za chikhalidwe zimagawidwa m'magulu awiri, makamaka pulasitiki ndi galasi.Galasiyo itha kugwiritsidwa ntchito ngati zinthu zakubzala, chikhalidwe cha tizilombo, komanso chikhalidwe chotsatira cha maselo a nyama.Zida zamapulasitiki zitha kukhala zida za polyethylene, zomwe zitha kugwiritsidwa ntchito kamodzi kapena kangapo.Iwo ndi abwino kwa labotale inoculation, scribing, ndi mabakiteriya kupatukana ntchito, ndipo angagwiritsidwe ntchito kulima zipangizo zomera.
Chifukwa chiyani mbale ya Petri ili mozondoka mu chikhalidwe cha lithographic——
1. Panthawi yogwira ntchito, pangakhale madontho a madzi kapena mabakiteriya pachivundikiro cha mbale ya petri.Chikhalidwe chozondoka chingalepheretse madontho amadzi kapena tizilombo toyambitsa matenda pachivundikiro kuti zisagwere pa mbale ya petri.
2. Panthawi ya chikhalidwe, mabakiteriya adzatulutsa metabolites ena owopsa pakukula ndi kubereka kwa mabakiteriya panthawi ya kubereka kwa metabolic, kumasula kutentha ndi kutulutsa madzi.Ngati mabakiteriya sanapangidwe mozondoka, madontho amadzi amagwera mu chikhalidwe cha chikhalidwe, zomwe zimakhudza kukula kwa midzi.
3. Ngati cholinga cha chikhalidwe ndi kusonkhanitsa bakiteriya metabolites, ndi metabolites mosavuta sungunuka m'madzi, inverted chikhalidwe atsogolere kusonkhanitsa.
Pa chikhalidwe, padzakhala nthunzi yambiri yamadzi mu mbale ya chikhalidwe, ndipo condensation ya nthunzi yamadzi pa chivundikiro cha mbale idzatulutsa madontho a madzi.Ngati mbale ya chikhalidwe imayikidwa pamalo abwino, madontho amadzi adzabalalitsa midzi.Mwanjira imeneyi, gulu lalikulu likhoza kumwazikana m'magulu ang'onoang'ono ambiri, zomwe zimayambitsa vuto lalikulu la kulima ndi kuwerengera mabakiteriya.Ngati zichitika, chikhalidwe cha chikhalidwe chimakhala pamwamba ndipo mbaleyo ili pansi pa chivundikirocho, ndipo madontho a madzi sangagwere pagulu.
Kusamala pakugwiritsa ntchito mbale za Petri——
1. Pambuyo poyeretsa ndi kupha tizilombo toyambitsa matenda musanagwiritse ntchito, ukhondo wa mbale za chikhalidwe zimakhudza kwambiri ntchito, zomwe zingakhudze pH ya chikhalidwe cha chikhalidwe.Ngati pali mankhwala ena, amalepheretsa kukula kwa mabakiteriya.
2. Zakudya zachikhalidwe zomwe zangogulidwa kumene ziyenera kutsukidwa ndi madzi otentha poyamba, ndikumizidwa mu 1% kapena 2% hydrochloric acid solution kwa maola angapo kuti muchotse zinthu zamchere zaulere, ndikutsukidwa kawiri ndi madzi osungunuka.
3. Kukulitsa mabakiteriya, gwiritsani ntchito nthunzi yothamanga kwambiri (kawirikawiri 6.8 * 10 Pa nthunzi yothamanga kwambiri mpaka mphamvu yachisanu), sungani kwa 30min pa 120 ℃, iumeni kutentha, kapena gwiritsani ntchito kutentha kouma kuti musatenthe, ndiko kuti, kuika. chikhalidwe mbale mu uvuni, kulamulira kutentha pa 120 ℃ kwa 2h, ndiyeno kupha mano bakiteriya.
4. Ndi mbale zosawilitsidwa zachikhalidwe zokha zomwe zingagwiritsidwe ntchito pobaya ndi kulima.
Chifukwa chiyani mbale ya Petri ili mozondoka mu chikhalidwe cha lithographic——
1. Panthawi yogwira ntchito, pangakhale madontho a madzi kapena mabakiteriya pachivundikiro cha mbale ya petri.Chikhalidwe chozondoka chingalepheretse madontho amadzi kapena tizilombo toyambitsa matenda pachivundikiro kuti zisagwere pa mbale ya petri.
2. Panthawi ya chikhalidwe, mabakiteriya adzatulutsa metabolites ena owopsa pakukula ndi kubereka kwa mabakiteriya panthawi ya kubereka kwa metabolic, kumasula kutentha ndi kutulutsa madzi.Ngati mabakiteriya sanapangidwe mozondoka, madontho amadzi amagwera mu chikhalidwe cha chikhalidwe, zomwe zimakhudza kukula kwa midzi.
3. Ngati cholinga cha chikhalidwe ndi kusonkhanitsa bakiteriya metabolites, ndi metabolites mosavuta sungunuka m'madzi, inverted chikhalidwe atsogolere kusonkhanitsa.
Pa chikhalidwe, padzakhala nthunzi yambiri yamadzi mu mbale ya chikhalidwe, ndipo condensation ya nthunzi yamadzi pa chivundikiro cha mbale idzatulutsa madontho a madzi.Ngati mbale ya chikhalidwe imayikidwa pamalo abwino, madontho amadzi adzabalalitsa midzi.Mwanjira imeneyi, gulu lalikulu likhoza kumwazikana m'magulu ang'onoang'ono ambiri, zomwe zimayambitsa vuto lalikulu la kulima ndi kuwerengera mabakiteriya.Ngati zichitika, chikhalidwe cha chikhalidwe chimakhala pamwamba ndipo mbaleyo ili pansi pa chivundikirocho, ndipo madontho a madzi sangagwere pagulu.
Nthawi yotumiza: Sep-20-2022