Kusamala kugwiritsa ntchito mbale za Petri
Kuyeretsa mbale za Petri
1. Kuviika: Zilowerereni magalasi atsopano kapena ogwiritsidwa ntchito ndi madzi oyera kuti mufewetse ndi kusungunula chomatacho.Musanagwiritse ntchito magalasi atsopano, ingotsukani ndi madzi apampopi, ndiyeno zilowerereni mu 5% hydrochloric acid usiku wonse;Magalasi omwe amagwiritsidwa ntchito nthawi zambiri amakhala ndi mapuloteni ambiri ndi mafuta, omwe si ophweka kupukuta pambuyo poyanika, choncho ayenera kumizidwa m'madzi oyera mwamsanga mukangogwiritsa ntchito potsuka.
2. Kutsuka: ikani magalasi oviikidwa m'madzi otsukira ndikutsuka mobwerezabwereza ndi burashi yofewa.Osasiya ngodya zakufa ndikuletsa kuwonongeka kwa kumapeto kwa zotengera.Sambani ndi kuyanika kutsukidwa glassware kwa pickling.
3. Pickling: Pickling ndi kuviika ziwiya zomwe zili pamwambazi muzitsulo zoyeretsera, zomwe zimadziwikanso kuti acid solution, kuchotsa zotsalira zomwe zingatheke pamwamba pa zotengera pogwiritsa ntchito okosijeni wamphamvu wa asidi.Kukolola sikuyenera kuchepera maola asanu ndi limodzi, nthawi zambiri usiku kapena kupitilira apo.Samalani poyika ndi kutenga ziwiya.
4. Kutsuka: Ziwiya zikatha kutsuka ndi pickling ziyenera kutsukidwa ndi madzi.Kaya ziwiya osambitsidwa oyera pambuyo pickling mwachindunji zimakhudza bwino kapena kulephera kwa selo chikhalidwe.Pakutsuka pamanja ziwiya zoziziritsa kukhosi, chotengera chilichonse chimayenera kudzazidwa mobwerezabwereza "madzi - kukhuthulidwa" kwa nthawi zosachepera 15, ndipo pamapeto pake kutsukidwa kwanthawi 2-3 ndi madzi otentha, owuma kapena owuma, ndikulongedza poyimirira.
5. Zakudya za pulasitiki zotayidwa nthawi zambiri zimakhala zosawilitsidwa kapena kusautsidwa ndi mankhwala zikachoka kufakitale.
Gulu la Petri mbale
2. Ikhoza kugawidwa mu mbale za pulasitiki za petri ndi mbale zagalasi za petri malinga ndi zipangizo zosiyanasiyana zopangira, koma mbale zonse za petri zomwe zimatumizidwa kunja ndi mbale zowonongeka ndi pulasitiki.
3. Malingana ndi kukula kwake, amatha kugawidwa kukhala 35mm, 60mm ndi 90mm m'mimba mwake.150mm Petri mbale.
4. Malingana ndi magawo osiyanasiyana, akhoza kugawidwa m'magulu awiri a Petri, 3 mbale za Petri, ndi zina zotero.
5. Zida za mbale za chikhalidwe zimagawidwa m'magulu awiri, makamaka pulasitiki ndi galasi.Galasi angagwiritsidwe ntchito zipangizo zomera, tizilombo chikhalidwe ndi adherent chikhalidwe cha nyama maselo.Zida zamapulasitiki zitha kukhala zida za polyethylene, zomwe zitha kugwiritsidwa ntchito kamodzi kapena kangapo.Iwo ndi abwino kwa labotale inoculation, scribing, ndi mabakiteriya kupatukana ntchito, ndipo angagwiritsidwe ntchito kulima zipangizo zomera.
Kusamala kugwiritsa ntchito mbale za Petri
1. Mbale ya chikhalidwe imatsukidwa ndi kutetezedwa ndi tizilombo tisanagwiritse ntchito.Kaya ndi yoyera kapena ayi imakhudza kwambiri ntchito, zomwe zingakhudze pH ya chikhalidwe cha chikhalidwe.Ngati mankhwala ena alipo, amalepheretsa kukula kwa mabakiteriya.
2. Zakudya zachikhalidwe zomwe zangogulidwa kumene ziyenera kutsukidwa ndi madzi otentha poyamba, kenaka kumizidwa mu 1% kapena 2% hydrochloric acid solution kwa maola angapo kuti muchotse zinthu zamchere zaulere, ndikutsukidwa kawiri ndi madzi osungunuka.
3. Kukulitsa mabakiteriya, gwiritsani ntchito nthunzi yothamanga kwambiri (nthawi zambiri 6.8 * 10 Pa nthunzi yothamanga kwambiri mpaka 5 mphamvu), ikani 30min pa 120 ℃, iumeni kutentha, kapena gwiritsani ntchito kutentha kouma kuti muyipitse, ndiko kuti, ikani mbale ya chikhalidwe mu uvuni, sungani kwa 2h pa 120 ℃, ndiyeno kupha mano a bakiteriya.
4. Chosabala mbale zachikhalidwe zitha kugwiritsidwa ntchito pobaya ndi kulima.
Nthawi yotumiza: Dec-28-2022