Zitsanzo zosonkhanitsira, zosungirako ndi zoyendetsa zoyeserera wamba

Zitsanzo zosonkhanitsira, zosungirako ndi zoyendetsa zoyeserera wamba

1. Kusonkhanitsa ndi kusunga zitsanzo za matenda:

☛Chigawo chozizira: Chotsani midadada yoyenera ndikuyisunga mu nayitrogeni wamadzimadzi;

☛Kugawa kwa Parafini: Chotsani matishu oyenerera ndikusunga mu 4% paraformaldehyde;

☛Masilayidi a ma cell: Ma slide a cell anaikidwa mu 4% paraformaldehyde kwa mphindi 30, kenaka analowetsedwa ndi PBS ndi kumizidwa mu PBS ndi kusungidwa pa 4°C.

2. Kusonkhanitsa ndi kusunga zitsanzo za biology ya mamolekyulu:

☛Minofu yatsopano: Dulani chitsanzocho ndikuchisunga mumadzi a nayitrogeni kapena -80°C mufiriji;

☛Zitsanzo za Parafini: Sungani kutentha kwa chipinda;

☛Chitsanzo chamagazi athunthu: Tengani kuchuluka koyenera kwa magazi athunthu ndikuwonjezera EDTA kapena heparin anticoagulation totole magazi chubu;

☛Zitsanzo zamadzimadzi a m'thupi: centrifugation yothamanga kwambiri kuti mutenge matope;

☛Zitsanzo za ma cell: Maselo amapangidwa lysed ndi TRizol ndi kusungidwa mu madzi nitrogen kapena -80°C mufiriji.

3. Kusonkhanitsa ndi kusungirako zoyeserera zama protein:

☛Minofu yatsopano: Dulani chitsanzocho ndikuchisunga mumadzi a nayitrogeni kapena -80°C mufiriji;

☛Chitsanzo chamagazi athunthu: Tengani kuchuluka koyenera kwa magazi athunthu ndikuwonjezera EDTA kapena heparin anticoagulation totole magazi chubu;

☛Zitsanzo za ma cell: Maselo amayeretsedwa mokwanira ndi cell lysis solution ndiyeno amasungidwa mumadzi a nitrogen kapena -80°C mufiriji.

4. Kusonkhanitsa ndi kusungirako ELISA, radioimmunoassay, ndi zitsanzo zoyesera za biochemical:

☛Zitsanzo za Seramu (plasma): Tengani magazi athunthu ndikuwawonjezera ku chubu chotchedwa procoagulation chubu (anticoagulation chubu), centrifuge pa 2500 rpm kwa mphindi pafupifupi 20, sonkhanitsani za supernatant, ndi kuzisunga mu madzi nitrogen kapena mufiriji -80°C;

☛Chitsanzo cha mkodzo: centrifuge chitsanzo pa 2500 rpm kwa mphindi pafupifupi 20, ndikusunga mumadzi a nitrogen kapena -80 ° C mufiriji;tchulani njira iyi ya thoracic ndi ascites fluid, cerebrospinal fluid, ndi alveolar lavage fluid;

☛Zitsanzo zamaselo: Mukazindikira zigawo zobisika, centrifuge zitsanzo pa 2500 rpm kwa mphindi pafupifupi 20 ndikuzisunga mumadzi wa nayitrogeni kapena -80°C mufiriji;pozindikira zigawo za intracellular, Dilute kuyimitsidwa kwa selo ndi PBS ndikuundana ndi kusungunuka mobwerezabwereza kuwononga maselo ndi kumasula zigawo za intracellular.Centrifuge pa 2500 rpm kwa mphindi pafupifupi 20 ndikusonkhanitsa supernatant monga pamwambapa;

☛Zitsanzo za minofu: Mukadula zitsanzozo, ziyeseni ndi kuziundana mumadzi a nayitrogeni kapena -80°C mufiriji kuti mudzagwiritse ntchito mtsogolo.

5. Zosonkhanitsira zitsanzo za Metabolomics:

☛Chitsanzo cha mkodzo: Centrifuge chitsanzo pa 2500 rpm kwa mphindi pafupifupi 20 ndikusunga mu nitrogen yamadzimadzi kapena -80 ° C mufiriji;kutanthauza njira imeneyi kwa thoracic ndi ascites madzimadzi, cerebrospinal madzimadzi, alveolar lavage madzimadzi, etc.;

☛Mukadula chitsanzo cha minofu, muyeseni ndikuchiwumitsa mumadzi a nayitrogeni kapena mufiriji -80°C kuti mudzagwiritse ntchito mtsogolo;