Kusankhidwa kwa cell Culture plate

Ma mbale a chikhalidwe cha selo amatha kugawidwa kukhala pansi pamunsi ndi kuzungulira pansi (U-woboola ndi V-woboola pakati) malinga ndi mawonekedwe a pansi;Chiwerengero cha mabowo a chikhalidwe chinali 6, 12, 24, 48, 96, 384, 1536, ndi zina zotero;Malinga ndi zida zosiyanasiyana, pali mbale ya Terasaki ndi mbale wamba yama cell.Kusankhidwa kwapadera kumadalira mtundu wa maselo otukuka, kuchuluka kwa chikhalidwe chofunikira ndi zolinga zosiyana zoyesera.

(1) Kusiyana ndi kusankhidwa kwa mbale zachikhalidwe zapansi ndi zozungulira (zooneka ngati U ndi V).

Maonekedwe osiyanasiyana a mbale zachikhalidwe ali ndi ntchito zosiyanasiyana.Ma cell a chikhalidwe nthawi zambiri amakhala a lathyathyathya, omwe ndi osavuta kuwonera, okhala ndi malo omveka bwino komanso mulingo wamadzimadzi amtundu wa cell.Choncho, pochita MTT ndi zoyesera zina, mbale yapansi yapansi imagwiritsidwa ntchito nthawi zambiri, mosasamala kanthu kuti maselo amamangiriridwa ku khoma kapena kuyimitsidwa.Chophimba chapansi chapansi panthaka chiyenera kugwiritsidwa ntchito kuyeza kuchuluka kwa kuyamwa.Samalani kwambiri pazinthuzo, ndipo lembani "Tissue Culture (TC) Treated" pa chikhalidwe cha maselo.

Ma mbale okhala ngati U kapena V nthawi zambiri amagwiritsidwa ntchito pazinthu zina zapadera.Mwachitsanzo, mu immunology, pamene ma lymphocyte awiri osiyana asakanizidwa ndi chikhalidwe, ayenera kukhudzana ndi kulimbikitsana.Panthawiyi, mbale zooneka ngati U zimagwiritsidwa ntchito chifukwa maselo amasonkhana pang'ono chifukwa cha mphamvu yokoka.Mbale yozungulira pansi yachikhalidwe itha kugwiritsidwanso ntchito kuyesa kuphatikizika kwa isotopu, komwe kumafunikira chida chosonkhanitsira ma cell kuti asonkhanitse chikhalidwe cha cell, monga "mixed lymphocyte culture".Ma mbale ooneka ngati V nthawi zambiri amagwiritsidwa ntchito poyesa kupha ma cell komanso kuyesa magazi a immunological agglutination.Kuyesera kwa kupha maselo kungasinthidwenso ndi mbale yooneka ngati U (pambuyo powonjezera ma cell, centrifuge pa liwiro lotsika).

(2) Kusiyana pakati pa mbale ya Terasaki ndi mbale wamba yama cell

Terasaki mbale makamaka ntchito crystallographic kafukufuku.Mapangidwe azinthu ndi osavuta kuyang'ana kristalo ndi kusanthula kapangidwe kake.Pali njira ziwiri: kukhala pansi ndi kupachika dontho.Njira ziwirizi zimagwiritsa ntchito masinthidwe osiyanasiyana azinthu.Crystal class polymer imasankhidwa ngati zinthu, ndipo zida zapadera ndizoyenera kuyang'ana mawonekedwe a kristalo.

Chimbale cha chikhalidwe cha cell chimapangidwa makamaka ndi zinthu za PS, ndipo zinthuzo zimasamalidwa pamwamba, zomwe zimakhala zosavuta kukula ndikukula kwa cell.Kumene, palinso zipangizo kukula kwa maselo planktonic, komanso otsika kumanga pamwamba.

(3) Kusiyana pakati pa mbale ya cell culture ndi mbale ya Elisa

Mbale ya Elisa nthawi zambiri imakhala yokwera mtengo kuposa mbale yama cell.Chophimba cha selo chimagwiritsidwa ntchito makamaka pa chikhalidwe cha maselo ndipo chingagwiritsidwenso ntchito kuyeza kuchuluka kwa mapuloteni;Mbale ya Elisa imaphatikizapo mbale zokutira ndi mbale zochitira, ndipo nthawi zambiri siziyenera kugwiritsidwa ntchito pa chikhalidwe cha ma cell.Amagwiritsidwa ntchito kwambiri pozindikira mapuloteni pambuyo polumikizana ndi ma enzyme, omwe amafunikira zofunikira zapamwamba komanso yankho lapadera la enzyme.

(4) Malo otsekera pansi ndi muyezo wamadzimadzi womwe umagwiritsidwa ntchito nthawi zambiri

Mulingo wamadzimadzi amadzimadzi omwe amawonjezedwa pama mbale osiyanasiyana otuluka sayenera kukhala ozama kwambiri, nthawi zambiri mkati mwa 2 ~ 3mm.Zoyenera zamadzimadzi kuchuluka kwa dzenje lililonse chikhalidwe akhoza masamu ndi kaphatikizidwe pansi m'dera la mabowo osiyana.Ngati madzi ochulukirapo awonjezeredwa, kusinthana kwa mpweya (oxygen) kudzakhudzidwa, ndipo kumakhala kosavuta kusefukira panthawi yosuntha, kuchititsa kuipitsa.Kuchulukana kwa ma cell kumatengera cholinga cha kuyesako.