Masitepe Enieni a Cell Culture

1. Zida wamba

1. Zida mu chipinda chokonzekera

Single distilled water distiller, double distilled water distiller, acid tank, uvuni, pressure cooker, kabati yosungiramo zinthu (zosungiramo zinthu zosaseweretsa), kabati yosungiramo (kusunga zinthu zosawilitsidwa), tebulo lonyamula.Zipangizo mu chipinda chokonzekera yankho: torsion balance ndi electro balance (kuyezera mankhwala), PH mita (kuyeza PH mtengo wa chikhalidwe yankho), maginito stirrer (kukonza njira yothetsera chipwirikiti yankho).

2. Zida za chipinda cha chikhalidwe

Tanki ya nayitrogeni yamadzimadzi, kabati yosungiramo (zosungirako zosungirako), nyali ya fulorosenti ndi nyali ya ultraviolet, makina oyeretsa mpweya, firiji yotentha kwambiri (- 80 ℃), air conditioner, carbon dioxide cylinder, side table (zolemba zolembera).

3. Zida zomwe ziyenera kuikidwa m'chipinda chosabala

Centrifuge (maselo otolera), ultra-clean worktable, inverted microscope, CO2 incubator (incubating chikhalidwe), kusamba m'madzi, makina atatu ophera ma oxygen ndi otsekereza, 4 ℃ firiji (kuyika njira ya seramu ndi chikhalidwe).

2, Aseptic opaleshoni

(1) Kutsekereza chipinda chosabala

1. Tsukani chipinda chosabala nthawi zonse: kamodzi pa sabata, choyamba gwiritsani ntchito madzi apampopi kuti mukolope pansi, pukutani tebulo, ndi kuyeretsa tebulo logwirira ntchito, ndiyeno mugwiritseni ntchito 3 ‰ lysol kapena bromogeramine kapena 0,5% peracetic acid kuti mupukute.

2. Kutseketsa kwa CO2 chofungatira (chofungatira): choyamba pukutani ndi 3 ‰ bromogeramine, ndiye pukutani ndi 75% mowa kapena 0,5% peracetic acid, ndiyeno irradiate ndi nyali ya ultraviolet.

3. Kutsekereza musanayambe kuyesa: yatsani nyali ya ultraviolet, chowumitsa ma oxygen atatu ndi makina oyeretsa mpweya kwa mphindi 20-30 motsatana.

4. Kutsekereza pambuyo kuyesera: misozi kopitilira muyeso-oyera tebulo, mbali tebulo ndi inverted maikulosikopu siteji ndi 75% mowa (3 ‰ bromogeramine).

Kutsekereza kukonzekera kwa labotale

1. Sambani m'manja ndi sopo.

2. Valani zovala zodzipatula, zipewa zodzipatula, masks ndi masilipper.

3. Pukutani m'manja ndi 75% ya mowa wa thonje mpira.

Chiwonetsero cha ntchito yosabala

1. Mabotolo onse a mowa, PBS, sing'anga ya chikhalidwe ndi trypsin omwe abweretsedwa mu benchi yoyeretsera kwambiri ayenera kupukuta ndi mowa wa 75% kunja kwa botolo.

2. Gwirani ntchito pafupi ndi moto wa nyali ya mowa.

3. Ziwiya ziyenera kutsekedwa musanagwiritse ntchito.

4. Ziwiya (monga zisoti za mabotolo ndi zotsitsa) zomwe zikupitiriza kugwiritsidwa ntchito ziyenera kuikidwa pamalo apamwamba, ndipo ziyenera kutenthedwabe panthawi yogwiritsira ntchito.

5. Ntchito zonse ziyenera kukhala pafupi ndi nyali ya mowa, ndipo ntchitoyo ikhale yopepuka komanso yolondola, ndipo sayenera kukhudzidwa mwachisawawa.Ngati udzu sungathe kukhudza thanki yamadzi otayika.

6. Mukafuna mitundu yopitilira iwiri yamadzimadzi, samalani ndikusintha chitoliro choyamwa kuti mupewe kuipitsidwa.

Onani mutu wotsatira wa zida zophera tizilombo.