Gawo loyamba pakuyesa kopambana kwa ELISA-kusankha mbale yoyenera ya ELISA

TheELISAmbale ndi chida chofunikira kwambiri cha ELISA, kuyesa kwa ma enzyme olumikizidwa ndi immunosorbent.Pali zinthu zambiri zomwe zimakhudza kupambana kwa kuyesa kwa ELISA.Kusankha chida choyenera ndi sitepe yoyamba.Kusankha microplate yoyenera kumathandizira kuyesa kuchita bwino.

Zinthu zaELISAmbale nthawi zambiri polystyrene (PS), ndipo polystyrene ali ndi kusakhazikika bwino kwa mankhwala ndipo akhoza kusungunuka ndi zosiyanasiyana zosungunulira organic (monga onunkhira ma hydrocarbons, halogenated hydrocarbons, etc.), ndipo akhoza dzimbiri ndi zidulo amphamvu ndi alkalis.Osagonjetsedwa ndi mafuta komanso osinthika mosavuta atakumana ndi kuwala kwa UV.

Mitundu yanjiELISAmbale zilipo?

✦Sankhani ndi mtundu

Transparent plate:oyenera kachulukidwe ndi qualitative olimba-gawo immunoassays ndi kumanga assays;

mbale yoyera:oyenera kudzipangira luminescence ndi chemiluminescence;

Mbale yakuda:oyenera ma immunoassays a fulorosenti ndi zoyeserera zomangira.

✦Sankhani pomanga mphamvu

Mbale yomangiriza pang'ono:Amamanga pang'onopang'ono ku mapuloteni kudzera pamtundu wa hydrophobic.Ndiwoyenera ngati chonyamulira cholimba cha ma macromolecular protein okhala ndi molekyulu yolemera> 20kD.Mphamvu yake yomanga mapuloteni ndi 200 ~ 300ng IgG/cm2.

mbale yomangiriza kwambiri:Pambuyo pa chithandizo chapamwamba, mphamvu yake yomanga mapuloteni imakula kwambiri, kufika 300 ~ 400ng IgG/cm2, ndipo kulemera kwa molekyulu ya mapuloteni akuluakulu ndi> 10kD.

✦Sanjani ndi mawonekedwe apansi

Pansi Pansi:low refractive index, yoyenera kudziwika ndi owerenga ma microplate;

U pansi:Mndandanda wa refractive ndiwokwera, womwe ndi wosavuta kuwonjezera, kukhumba, kusakaniza ndi ntchito zina.Mutha kuwona mwachindunji kusintha kwamtundu poyang'ana popanda kuziyika pa owerenga ma microplate kuti muwone ngati pali chitetezo chokwanira.