Malangizo pakusankha ndi kugwiritsa ntchito mbale za cell culture (I)
Monga chida chodziwika bwino komanso chofunikira cha chikhalidwe cha ma cell, mbale ya cell culture ili ndi mawonekedwe osiyanasiyana, mawonekedwe ndi ntchito.
Kodi mwasokonezekanso momwe mungasankhire mbale yoyenera yachikhalidwe?
Mukuda nkhawa ndi momwe mungagwiritsire ntchito mbale yachikhalidwe mosavuta komanso moyenera?
Kodi mwasokonezeka momwe mungachitire ndi mbale yachikhalidwe?
Kodi mumamva bwanji mukamagwiritsa ntchito bwino mbale zamitundu yosiyanasiyana?
Kodi kusankha mbale chikhalidwe cell?
1) Mabala amtundu wa cell amatha kugawidwa kukhala pansi pamunsi ndi kuzungulira pansi (U-woboola pakati ndi V-woboola pakati) malinga ndi mawonekedwe a pansi;
2) Chiwerengero cha mabowo a chikhalidwe chinali 6, 12, 24, 48, 96, 384, 1536, etc;
3) Malinga ndi zida zosiyanasiyana, pali mbale ya Terasaki ndi mbale wamba yama cell.Kusankhidwa kwapadera kumadalira mtundu wa maselo otukuka, kuchuluka kwa chikhalidwe chofunikira ndi zolinga zosiyana zoyesera.
Kusiyana ndi kusankhidwa kwa mbale zachikhalidwe zapansi ndi zozungulira (zooneka ngati U-zooneka ngati V).
Mitundu yosiyanasiyana ya matabwa mwachilengedwe imakhala ndi ntchito zosiyanasiyana
Mitundu yonse ya maselo apansi apansi angagwiritsidwe ntchito, koma pamene chiwerengero cha maselo ndi ochepa, monga cloning, 96 bwino pansi lathyathyathya mbale pansi ntchito.
Kuphatikiza apo, pochita MTT ndi zoyeserera zina, mbale yapansi yapansi nthawi zambiri imagwiritsidwa ntchito pama cell omwe amamatira komanso oimitsidwa.
Ponena za mbale zooneka ngati U kapena V, nthawi zambiri zimagwiritsidwa ntchito pazinthu zina zapadera.Mwachitsanzo, mu immunology, pamene ma lymphocyte awiri osiyana asakanizidwa, ayenera kulumikizana wina ndi mzake kuti alimbikitse.Chifukwa chake, mbale zooneka ngati U nthawi zambiri zimafunikira.Chifukwa ma cell amasonkhana pang'ono chifukwa cha mphamvu yokoka, mbale zooneka ngati V sizothandiza kwenikweni.Ma mbale opangidwa ndi V nthawi zambiri amagwiritsidwa ntchito poyesera kupha maselo kuti apange maselo omwe akutsata kuti agwirizane kwambiri, koma mbale zooneka ngati U zitha kugwiritsidwanso ntchito pakuyesa uku (pambuyo powonjezera ma cell, centrifuge pa liwiro lotsika).
Ngati imagwiritsidwa ntchito pa chikhalidwe cha ma cell, nthawi zambiri imakhala yotsika pansi.Kuphatikiza apo, chidwi chapadera chiyenera kuperekedwa kuzinthuzo.Chizindikiro "Tissue Culture (TC) Treated" chimagwiritsidwa ntchito pa chikhalidwe cha ma cell.
Pansi yozungulira nthawi zambiri imagwiritsidwa ntchito posanthula, kuchitapo kwa mankhwala, kapena kusunga zitsanzo.Chifukwa zozungulira m'munsi ndi bwino kuyamwa zamadzimadzi, ndipo pansi lathyathyathya si.Komabe, ngati mukufuna kuyeza kuchuluka kwa kuyamwa kwa kuwala, muyenera kugula pansi.
Zikhalidwe zambiri zama cell zimagwiritsa ntchito mbale zapansi, zomwe zimakhala zosavuta kuziwona pansi pa maikulosikopu, zimakhala ndi malo omveka bwino, zimakhala ndi msinkhu wamadzimadzi amtundu wa cell, komanso zimathandizira kuzindikira kwa MTT.
Chimbale chozungulira chapansi cha chikhalidwe chimagwiritsidwa ntchito makamaka kuyesa kuphatikizika kwa isotopu, ndipo chida chosonkhanitsira ma cell chimafunika kusonkhanitsa chikhalidwe cha cell, monga "mixed lymphocyte culture".
Nthawi yotumiza: Dec-08-2022