Gwiritsani Ntchito Njira ndi Kupewa Kuzizira kwa Tube
M'mayesero a microbiological, chida chimodzi choyesera chimagwiritsidwa ntchito nthawi zambiri, ndiko kuti, chubu cha cryopreservation.Komabe, chifukwa cha zovuta zake zosiyanasiyana, zotsatira zake zimasiyana kwambiri.Pachifukwachi, pakali pano, ma laboratories ambiri ku China amapanga machubu osungira mabakiteriya okha, omwe samangowonjezera mphamvu ya ntchito, komanso chifukwa cha zofooka zamitundu yosiyanasiyana, zotsatira za kusunga mabakiteriya sizikhala zokhutiritsa nthawi zonse.
Chifukwa chake, ndikofunikira kudziwa bwino njira yogwiritsira ntchito komanso njira zina zodzitetezera ku chubu cha cryopreservation, kuti mutenge gawo lalikulu.
1.Njira yogwiritsira ntchito
1).Mukamagwiritsa ntchito chubu cha cryopreservation kuti musunge zitsanzo, ndikofunikira kuyika chubu cha cryopreservation mu nthunzi wosanjikiza wa nayitrogeni wamadzimadzi kapena mufiriji kuti musungidwe.Ngati chubu cha cryopreservation chasungidwa mu nayitrogeni wamadzimadzi, pali kuthekera kuti nayitrogeni wamadzimadzi alowerere mu chubu cha cryopreservation.Pakuchira, mpweya wa nayitrogeni wamadzimadzi umayambitsa kusalinganika kwapakati ndi kunja, zomwe zimatha kuyambitsa chubu cha cryopreservation kuphulika, ndipo zimakhala ndi zoopsa zamoyo.
2).Gwiritsani ntchito chubu cha cryopreservation kuti mutsitsimutse, ndikugwiritsa ntchito zida zotetezera chitetezo panthawi yonseyi.Ndikoyenera kuvala zovala za labotale, magolovesi a thonje ndikugwira ntchito pa benchi yotetezeka ya labotale.Ngati n'kotheka, chonde valani magalasi kapena zoteteza kumaso.Popeza kutentha kwa m'nyumba m'chilimwe kudzakhala kokwera kuposa nthawi yozizira, chonde samalani.
3).Pakusungirako ma cell a cryopreserved, kutentha kozizira kwa machubu a cryopreserved kuyenera kukhala kofanana.Kuzizira kosagwirizana kumabweretsa kupanikizana kwa ayezi, komwe kumalepheretsa kufalikira kwa kutentha kwamadzi kumbali zonse ziwiri, motero kumayambitsa kuthamanga kwambiri ndikuwononga chubu lozizira.
4).Kuchuluka kwa zitsanzo zowuma sikudutsa kuchuluka kwazomwe zimafunidwa ndi chubu chachisanu.
2. Nkhani zofunika kuziganizira
1).Kuzizira kosungirako machubu
Machubu a cryopreservation osagwiritsidwa ntchito amatha kusungidwa firiji kapena 2-8 ℃ kwa miyezi 12;The inoculated cryopreservation chubu akhoza kusungidwa pa - 20 ℃ ndi kukhala ndi zotsatira zabwino kusungitsa mavuto mkati 12 miyezi;The inoculated cryopreservation chubu akhoza kusungidwa pa - 80 ℃, ndipo mavuto akhoza kusungidwa bwino mkati 24 miyezi.
2).Kuzizira nthawi yosungira machubu
Machubu a cryopreservation osagwiritsidwa ntchito amatha kusungidwa kutentha kwa chipinda kapena 2-8 ℃;The inoculated cryopreservation chubu ayenera kusungidwa pa -20 ℃ kapena - 80 ℃.
3).Njira zogwirira ntchito za chubu chozizira
Tengani zikhalidwe zatsopano kuchokera ku chikhalidwe choyera cha mabakiteriya kuti mukonzekere kuyimitsidwa kwa bakiteriya ndi turbidity pafupifupi 3-4 chiŵerengero cha McDonnell cha inoculation ndi kusungirako chubu;Limbikitsani chubu chosungira ndikuchisintha mmbuyo ndi mtsogolo maulendo 4-5 kuti mabakiteriya asungunuke, osazungulira;Ikani chubu chosungira mufiriji kuti chisungidwe (- 20 ℃ - 70 ℃
Nthawi yotumiza: Nov-25-2022