Chifukwa chiyani ma cryovials amaphulika?Kodi mungapewe bwanji?

Chifukwa chiyani ma cryovials amaphulika?Kodi mungapewe bwanji?

Pakuyesa, titha kugwiritsa ntchitomatenda a cryovialkuzizira zitsanzo, koma kuzizira ndi nayitrogeni wamadzi,matenda a cryovialnthawi zambiri zimaphulika, zomwe sizimangoyambitsa kutayika kwa zitsanzo zoyesera, koma zimatha kuyambitsa kuwonongeka kwa zitsanzo.Oyesera amawononga, ndiye mungapewe bwanji izi?

Zifukwa:

Choyambirira,matenda a cryovialsangathe kuyikidwa mwachindunji mu gawo lamadzimadzi la nayitrogeni wamadzimadzi kuti asungidwe.Chifukwa zinthu chubu thupi ndi kapu ya wambamatenda a cryovialndi zosiyana, mitengo ya kukula kwa matenthedwe ndi kutsika pa nthawi ya kuzizira ndi yosiyana.Ngati muyikacryovialmolunjika mu gawo lamadzimadzi, mutha kulola nayitrogeni wamadzimadzi kulowa mu chubu.Pamene resuscitating chitsanzo nthawi ina, ikanicryogenic - mbalemu osamba madzi pa 37 ° C, madzi asafe mu chubu mwamsanga vaporized ndi kukulitsa, koma mpweya sakanatha kuthawa chubu mu nthawi, zomwe zinachititsa cryopreservation chubu kuphulika.

Momwe mungapewere:

1. Osasungamatenda a cryovialmolunjika mu gawo lamadzimadzi, koma mu gawo la gasi.Kapena ingozizirani mwachindunji mufiriji.Kumbukirani kuti musayike molunjika pansi pa madzi a nayitrogeni.

2. Gwiritsani ntchito kuzungulira kwamkaticrotubes.

Kumene, ngakhale mkati zimazunguliracrotubessangathe kuyikidwa mwachindunji mu gawo lamadzimadzi, koma mkati mozunguliracrotubeskukhala ndi kulekerera kwabwinoko kwa kutentha kochepa kusiyana ndi zipewa zozungulira kunja, zomwe zingathe kuchepetsa kuphulika komanso zimakhala zotetezeka.Kuzungulira kwakunjacrystalkwenikweni lakonzedwa kuti mawotchi kuzizira, choncho si oyenera madzi asafe yosungirako.

3. Ndiye muyenera kuchita chiyani ngati mukufunadi kusunga mu gawo lamadzimadzi?Pofuna kuthana ndi vutoli, pali manja a cryopreservation chubu omwe amapangidwa mwapadera kuti achite izi, omwe angagwiritsidwe ntchito kusindikiza chubu cha cryopreservation ndikuchiyika mu gawo lamadzimadzi.Inde, mungagwiritsenso ntchito filimu yosindikizira, tepi yachipatala, ndi zina zotero kuti musindikize, kotero kuti sipadzakhalanso kuphulika.