Chifukwa chiyani chithandizo chapamwamba cha TC chikufunika pazakudya zama cell?
Pali mitundu yosiyanasiyana ya maselo, amene akhoza kugawidwa mu adherent maselo ndi kuyimitsidwa maselo mawu a chikhalidwe njira Maselo oyimitsidwa ndi maselo amakula paokha padziko thandizo, ndi kukula mu kuyimitsidwa mu sing'anga chikhalidwe, monga lymphocytes Adherent maselo. ndi maselo otsatizana, zomwe zikutanthauza kuti kukula kwa maselo kuyenera kukhala ndi gawo lothandizira.Amatha kukula ndi kuberekana pamtunda uwu podalira zinthu zomatira zomwe zimabisala okha kapena zoperekedwa mu chikhalidwe cha chikhalidwe.Maselo ambiri a nyama amakhala m'maselo ogwirizana.
Poyamba, ambiri a cell chikhalidwe consumables pa msika anapangidwa galasi, amene anali hydrophilic, kotero pamwamba sanali kufunika chithandizo chapadera Komabe, mu ndondomeko yeniyeni ntchito, pali zofooka zina monga chidetso ndi zosavuta kuipitsa chitsanzo Ndi. Kukula kwachangu kwa sayansi ndi ukadaulo, zida zosiyanasiyana za polima (monga polystyrene PS) zasintha pang'onopang'ono zida zamagalasi ndikukhala zida zoyambira zopangira zinthu zomwe zimagwiritsidwa ntchito pama cell.
Polystyrene ndi amorphous random polima ndi kuwonekera.Zogulitsa zake zimakhala zowonekera kwambiri, zomwe zimadutsa 90%, zomwe zimathandiza kuyang'ana chikhalidwe cha maselo pansi pa microscope.Kuonjezera apo, ili ndi ubwino wa mitundu yosavuta, yabwino yopangira madzimadzi, kukhazikika bwino komanso kukana kwa mankhwala opangira mankhwala Komabe, pamwamba pa polystyrene ndi hydrophobic.Pofuna kuonetsetsa kuti maselo otsatizana amatha kugwirizanitsa pamwamba pa zowonongeka bwino, pamwamba pa zinthu zomwe zimagwiritsidwa ntchito pamtundu wa selo ziyenera kuchitidwa chithandizo chapadera chosinthidwa.Zinthu za hydrophilic zimayambitsidwa pamtunda kuti zigwirizane ndi kukula ndi kubereka kwa maselo ogwirizana.Mankhwalawa amatchedwa TC surface treatment TC mankhwala amagwiritsidwa ntchito pa mbale za chikhalidwe cha ma cell, mbale zama cell, mbale zokwera ma cell, mabotolo amtundu wa cell, ndi zina zambiri, zida zochizira zam'madzi zam'madzi zimagwiritsidwa ntchito pokwaniritsa TC yochizira mbale zama cell.
Makhalidwe a cell culture mbale pambuyo pa chithandizo cha TC pamwamba
1. Kuyeretsa koyambirira kwa chinthucho: O2 plasma imatha kuyamwa tinthu ting'onoting'ono ndi zowononga zina zomwe zimayikidwa pamalopo, ndikutulutsa mpweya wosakanikirana kuchokera muchipinda chochotsera vacuum pampu kuti mukwaniritse kuyeretsa kusanachitike.
2. Kuchepetsa mavuto padziko mankhwala, kotero kuti madzi kukhudzana ngodya ya mankhwala yafupika, ndi zikugwirizana yoyenera ionization mphamvu ndi ndende, kuti madzi kukhudzana ngodya ya mankhwala pamwamba WCA<10 °.3 The O2 plasma idzachitapo kanthu pa mankhwala pamtunda, ndipo magulu ambiri ogwira ntchito amatha kuwonjezeredwa pamwamba pa mankhwala, kuphatikizapo hydroxyl (- OH), carboxyl (- COOH), carbonyl (- CO -), hydroperoxy (- OOH), etc. Magulu ogwira ntchitowa amatha kupititsa patsogolo liwiro la chikhalidwe ndi zochitika pa chikhalidwe cha selo.
Nthawi yotumiza: Jan-13-2023