Chifukwa chiyani chithandizo cha TC chikufunika pazakudya zama cell

Chifukwa Chake Tissue Culture Treated (TC Treated) ndiyofunika pazakudya zama cell

Pali mitundu yosiyanasiyana ya maselo, amene akhoza kugawidwa mu adherent maselo ndi kuyimitsidwa maselo mawu a chikhalidwe njira Maselo oyimitsidwa ndi maselo amakula paokha padziko thandizo, ndi kukula mu kuyimitsidwa mu sing'anga chikhalidwe, monga lymphocytes Adherent maselo. ndi maselo otsatizana, zomwe zikutanthauza kuti kukula kwa maselo kuyenera kukhala ndi gawo lothandizira.Amatha kukula ndi kuberekana pamtunda uwu podalira zinthu zomatira zomwe zimabisala okha kapena zoperekedwa mu chikhalidwe cha chikhalidwe.Maselo ambiri a nyama amakhala m'maselo ogwirizana

Poyamba, ambiri a cell chikhalidwe consumables pa msika anapangidwa galasi, amene anali hydrophilic, kotero pamwamba sanali kufunika chithandizo chapadera Komabe, mu ndondomeko yeniyeni ntchito, pali zofooka zina monga chidetso ndi zosavuta kuipitsa chitsanzo Ndi. Kukula mwachangu kwa sayansi ndi ukadaulo, zida zosiyanasiyana za polima (monga polystyrene PS) zasintha pang'onopang'ono zida zamagalasi ndikukhala zida zoyambira zopangira zinthu zogwiritsira ntchito zama cell.

Polystyrene ndi amorphous random polima ndi kuwonekera.Zogulitsa zake zimakhala zowonekera kwambiri, zomwe zimadutsa 90%, zomwe zimathandiza kuyang'ana chikhalidwe cha maselo pansi pa microscope.Kuphatikiza apo, ili ndi maubwino a utoto wosavuta, kusungunuka kwamadzimadzi, kukhazikika kwabwino komanso kukana kwa dzimbiri kwamankhwala.Komabe, pamwamba pa polystyrene ndi hydrophobic.Pofuna kuonetsetsa kuti maselo otsatizana amatha kugwirizanitsa pamwamba pa zowonongeka bwino, pamwamba pa zinthu zomwe zimagwiritsidwa ntchito pamtundu wa selo ziyenera kuchitidwa chithandizo chapadera chosinthidwa.Zinthu za hydrophilic zimayambitsidwa pamtunda kuti zigwirizane ndi kukula ndi kubereka kwa maselo ogwirizana.Mankhwalawa amatchedwa TC treatment.Mankhwala a TC amagwiritsidwa ntchito pazakudya zama cell, mbale zama cell, mbale zokwera ma cell, mabotolo amtundu wa cell, ndi zina zambiri, zida zochizira zam'madzi zam'madzi zimagwiritsidwa ntchito kuti TC isagwiritsidwe ndi mbale zama cell.

Makhalidwe a mbale ya cell culture pambuyo pa chithandizo cha TC:

1. Kuyeretsa koyambirira kwa mankhwala: O2 plasma imatha kuyamwa tinthu ting'onoting'ono ndi zowononga zina zomwe zimayikidwa pamwamba pa mankhwalawo, ndikujambula mpweya wosakanikirana kuchokera m'chipinda chosungiramo mpweya kudzera papampu ya vacuum kuti ikwaniritse kuyeretsa kusanachitike.